Huzzah® C1P
Human Serum Albumin/Ceramide-1-Phosphate Complex
Avanti’s new HSA lipid delivery system, Huzzah™, improves the cellular delivery of C1P through its conjugation with human serum albumin (HSA), a physiologically relevant carrier protein. This conjugate system eliminates the need for organic solvents, which are well-known to adversely affect viability and the phenotypic characteristics of cells. The Huzzah™ lipid delivery system is supplied as a lyophilized conjugate which can be directly and easily reconstituted in water or a buffer of choice and is ideal for studying the effects of poorly soluble bioactive molecules.
Solubility:Reconstitute lyophilized conjugate in 5 mL of water or other aqueous buffer. This produces a stock solution of 120 - 200 µM sphingosine-1-phosphate*. A short sonication may be necessary to ensure complete dissolution. If necessary, filter sterilize the reconstituted conjugate prior to use in cell culture applications. *Exact concentration will vary from batch to batch. Refer to label for exact product concentration upon reconstitution as determined by mass spectrometric analysis.
Molar Ratio:C1P:albumin (1:1)Albumin Biological Characterization:microbial free; endotoxin < 1 IU/mLAlbumin Lipid Characterization:~0.07g/L phospholipids; ~0.02 g/L sphingolipids; ~0.1 g/L fatty acids; and triglycerides and cholesterols below the limit of quantitation of 0.1 g/L and 0.05 g/L
